TAK1 Kinase Assay Service

Activity-based TAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
TAK1

Enzyme
Human TAK1

HGNC
MAP3K7

Synonym
TGF1a, MEKK7

Substrates
Casein, 1 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 36
Ro 31-8220 16,700
GW 5074 14,000
Scientific Information
O43318 (http://www.uniprot.org/uniprot/O43318)

Screening Location
Malvern, USA

Reference compound IC50 for TAK1
Reference compound IC50 for TAK1