Activity-based TAOK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TAOK1
Enzyme
Human TAOK1
HGNC
TAOK1
Synonym
TAO1, KIAA1361, MAP3K16, MARKK,
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.1 |
| Ro 31-8220 | 435 |
| GW 5074 | 5,300 |
| H-89 | 26,300 |
| Rottlerin | 36,500 |
Scientific Information
Q7L7X3 (http://www.uniprot.org/uniprot/Q7L7X3)
Screening Location
Malvern, USA
Reference compound IC50 for TAOK1
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