Activity-based TAOK2/TAO1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TAOK2
Enzyme
Human TAOK2
HGNC
TAOK2
Synonym
KIAA0881, MAP3K17, PSK, PSK1, TAO1, TAO2
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.6 |
| Ro 31-8220 | 238 |
| GW 5074 | 3,000 |
| H-89 | 27,600 |
| Rottlerin | 82,000 |
Scientific Information
Q9UL54 (http://www.uniprot.org/uniprot/Q9UL54)
Screening Location
Malvern, USA
Reference compound IC50 for TAOK2
PDF