Activity-based TAOK3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
TAOK3
Enzyme
Human TAOK3, wt
HGNC
TAOK3
Manning ID
TAO3
Synonym
DKFZp666H245, DPK, JIK, MAP3K18, TAO kinase 3, TAOK3, TAOK3/JIK
UniProt
Q9H2K8 (TAOK3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
3xChocktide-PKC(19-31), ATP
Reaction
3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 47 |
| Sorafenib | 470 |
| Sunitinb | 72000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | TAOK3 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – TAOK3 |
| Product Number |
0908-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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