Activity-based TESK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TESK2
Enzyme
Human TESK2
HGNC
TESK2
Synonym
TESK2
Substrates
Cofilin 2, 5 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 11,200 |
| Ro 31-8220 | 13,900 |
| GW 5074 | 2,600 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q96S53 (http://www.uniprot.org/uniprot/Q96S53)
Screening Location
Malvern, USA
Reference compound IC50 for TESK2
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