Activity-based TGFBR2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TGFβR2
Enzyme
Human TGFβR2
HGNC
TGFBR2
Synonym
HNPCC6, MFS2, RIIC, TGFbeta-RII
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 23,000 |
| Ro 31-8220 | >10,000 |
| GW 5074 | 8,300 |
| LDN193189 | 90 |
| Rottlerin | 34,000 |
Scientific Information
P37173 (http://www.uniprot.org/uniprot/P37173)
Screening Location
Malvern, USA
Reference compound IC50 for TGFβR2
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