Activity-based TIE2 (L914F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TIE2 (L914F)
Enzyme
Human TIE2 (L914F)
HGNC
TEK
Synonym
VMCM, TIE-2, VMCM1, CD202B
Scientific Information
Q02763 (http://www.uniprot.org/uniprot/Q02763)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
Cofactor: MnCl2, 2 mM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of TIE2 (L914F)
