TIE2 (A1124V) Kinase Assay Service

Activity-based TIE2 (A1124V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TIE2 (A1124V)
Enzyme
Human TIE2 (A1124V)
HGNC
TEK (A1124V)
Synonym
VMCM, TIE-2, VMCM1, CD202B
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine37
PP25,400
AG147813,000
PDGFR I-III35,000
D-6440621,000
Scientific Information
Q02763 (http://www.uniprot.org/uniprot/Q02763)
Screening Location
Malvern, USA
Reference compound IC50 for TIE2 (A1124V)
Reference compound IC50 for TIE2 (A1124V)