TIE2 (P883A) Kinase Assay Service

Activity-based TIE2 (P883A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
TIE2 (P883A)

Enzyme
Human TIE2 (P883A)

HGNC
TEK (P883A)

Synonym
VMCM, TIE-2, VMCM1, CD202B

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 39
PP2 4,200
AG1478 6,600
PDGFR I-III 24,000
D-64406 30,000
Scientific Information
Q02763 (http://www.uniprot.org/uniprot/Q02763)

Screening Location
Malvern, USA

Reference compound IC50 for TIE2 (P883A)
Reference compound IC50 for TIE2 (P883A)