Activity-based TIE2 (R915C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TIE2 (R915C)
Enzyme
Human TIE2 (R915C)
HGNC
TEK (R915C)
Synonym
Angiopoietin-1 receptor, Tyrosine-protein kinase receptor TIE-2, hTIE2, Tyrosine-protein kinase receptor TEK, p140 TEK, Tunica interna endothelial cell kinase
Scientific Information
Q02763 (http://www.uniprot.org/uniprot/Q02763)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 82 |
| PP2 | 40,000 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for TIE2 (R915C)
