TIE2 (Y897H/R915C) Kinase Assay Service

Activity-based TIE2 (Y897H/R915C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

TIE2 (Y897H/R915C)

Enzyme

Human TIE2 (Y897H/R915C)

HGNC

TEK (Y897H/R915C)

Synonym

Angiopoietin-1 receptor, Tyrosine-protein kinase receptor TIE-2, hTIE2, Tyrosine-protein kinase receptor TEK, p140 TEK, Tunica interna endothelial cell kinase

Scientific Information

Q02763 (http://www.uniprot.org/uniprot/Q02763)

Assay Properties

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 430
PP2 >100,000
AG1478 >100,000
EGFR/ErbB Inhibitor >100,000
D-64406 >100,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for TIE2 (Y897H/R915C)