TLK1 Kinase Assay Service - Reaction Biology

Activity-based TLK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

TLK1

Enzyme

Human TLK1

HGNC

TLK1

Synonym

PKU-beta, KIAA0137

Substrates

Histone H3, 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q9UKI8 (http://www.uniprot.org/uniprot/Q9UKI8)

Screening Location

Malvern, USA

Reference compound IC50 for TLK1