TNIK Kinase Assay Service - Reaction Biology

Activity-based TNIK kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

TNIK

Enzyme

Human TNIK

HGNC

TNIK

Synonym

TRAF2 and NCK interacting kinase, KIAA0551

Substrates

Peptide substrate, [RLGRDKYKTLRQIRQ], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q9UKE5 (http://www.uniprot.org/uniprot/Q9UKE5)

Screening Location

Malvern, USA

Reference compound IC50 for TNIK