TRIM33b Bromodomain Assay Service (ThermoShift)

TRIM33b-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-TRIM33b-H

Protein

Human TRIM33b bromodomain, His-tagged

Synonyms

E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; TIF1-gamma; Tripartite motif-containing protein 33

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.075 mg/ml (3.1 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

50,5

Reference Compound ΔTms:

Bromosporine - 1.0 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, GSK2801 and PFI3

Scientific Information

Q9UPN9 (http://www.uniprot.org/uniprot/Q9UPN9)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak