TRKA-TPR Kinase Assay Service

Activity-based TRKA-TPR kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

TRKA, TPR-

Enzyme

Human TPR-TRKA fusion

HGNC

NTRK1-TPR

Synonym

TRKA

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

TPR; P12270 (http://www.uniprot.org/uniprot/P12270)TRKA; P04629 (http://www.uniprot.org/uniprot/P04629)

Screening Location

Malvern, USA

Reference compound IC50 for TRKA-TPR