TRKA (G595R/G667A) Kinase Assay Service

Activity-based TRKA (G595R/G667A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TRKA (G595R/G667A)
Enzyme
Human TRKA (G595R/G667A)
HGNC
NTRK1 (G595R/G667A)
Synonym
MTC, TRK, TRK1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.2
PP23,900
AG1478>100,000
EGFR/Erb Inhibitor>100,000
D-6440613,000
Scientific Information
P04629 (http://www.uniprot.org/uniprot/P04629)
Screening Location
Malvern, USA
Reference compound IC50 for TRKA (G595R/G667A)
Reference compound IC50 for TRKA (G595R/G667A)