TRKB Kinase Assay Service

Activity-based TRKB kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
TRKB

Enzyme
Human TRKB

HGNC
NTRK2

Synonym
GP145-TrkB

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Scientific Information
Q16620 (http://www.uniprot.org/uniprot/Q16620)

Screening Location
Malvern, USA

Reference compound IC50 for TRKB