TRPM7/CHAK1 Kinase Assay Service

Activity-based TRPM7/CHAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TRPM7/CHAK1
Enzyme
Human TRPM7/CHAK1
HGNC
TRPM7
Synonym
CHAK1
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine>100,000
Ro 31-8220>100,000
GW507431,000
H-89>100,000
NH12514,000
Scientific Information
Q96QT4 (http://www.uniprot.org/uniprot/Q96QT4)
Screening Location
Malvern, USA
Reference compound IC50 for TRPM7/CHAK1
Reference compound IC50 for TRPM7/CHAK1