TSSK3/STK22C Kinase Assay Service

Activity-based TSSK3/STK22C kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TSSK3/STK22C
Enzyme
Human TSSK3/STK22C
HGNC
TSSK3
Synonym
STK22C, STK22D, SPOGA3
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine29
Ro 31-822014,000
GW 50745,500
H-89>50,000
Rottlerin>50,000
Scientific Information
Q96PN8 (http://www.uniprot.org/uniprot/Q96PN8)
Screening Location
Malvern, USA
Reference compound IC50 for TSSK3/STK22C
Reference compound IC50 for TSSK3/STK22C