VRK1 Kinase Assay Service

Activity-based VRK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
VRK1
Enzyme
Human VRK1
HGNC
VRK1
Synonym
VRK1
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine>100,000
Ro 31-8220720
GW 507457,000
H-8913,000
Rottlerin>100,000
Scientific Information
Q99986 (http://www.uniprot.org/uniprot/Q99986)
Screening Location
Malvern, USA
Reference compound IC50 for VRK1
Reference compound IC50 for VRK1
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