Activity-based WEE1 kinase assay for compound screening and profiling via Probe-Displacement kinase assay.
Target Overview
CAT#
WEE1 (Binding)
Enzyme
Human WEE1
HGNC
WEE1
Synonym
Wee1-like protein kinase, Wee1A kinase, WEE1hu
Assay Properties
Probe
Fluorescent Kinase Tracer based on ATP-competitive kinase inhibitors, 25 nM
Assay Format
Binding interaction between Fluorescent Kinase Tracer based and Kinase is quantified by HTRF technology using anti Tag HTRF reagents. Compounds binding in this binding pocket displace fluorescently labeled probe resulting loss of binding signal.
Readout
HTRF Readout, Probe-Displacement
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| WEE1 Inhibitor | 0.49 nM |
| Lunresertib (RP-6306) | 316 nM |
| Staurosporine | 1.1 µM |
Scientific Information
P30291 (http://www.uniprot.org/uniprot/P30291)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition for WEE1
