Activity-based WEE1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
WEE1
Enzyme
Human WEE1, wt
HGNC
WEE1
Manning ID
WEE1
Synonym
WEE1hu, WEE1
UniProt
P30291 (WEE1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1253 |
| Sorafenib | 10000 |
| Sunitinb | 4600 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | WEE1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – WEE1 |
| Product Number |
0136-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF