Activity-based WEE1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
WEE1
Enzyme
Human WEE1
HGNC
WEE1
Synonym
Wee1-like protein kinase, Wee1A kinase, WEE1hu
Substrates
Protein substrate, CDK1 (D146N)/cyclin B1, 5 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 6,500 |
| PP2 | >100,000 |
| AG1478 | >100,000 |
| WEE1 Inhibitor | 14 |
| D-64406 | >100,000 |
Scientific Information
P30291 (http://www.uniprot.org/uniprot/P30291)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for WEE1
