Activity-based WNK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
WNK1
Enzyme
Human WNK1
HGNC
WNK1
Synonym
KDP, KIAA0344, PHA2C, PRKWNK1
Assay Properties
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q9H4A3 (http://www.uniprot.org/uniprot/Q9H4A3)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of WNK1
