YTHDC1 Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.
Target Overview
CAT#
YTHDC1
Enzyme
Human YTHDC1 (Full Length, FLAG tag at N-terminal)
Synonyms
Splicing factor YT521, YT521-B, KIAA1966
Scientific Information
Q96MU7 (https://www.uniprot.org/uniprotkb/Q96MU7/entry)
Assay Properties
Ligand
Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’
Reaction
Binding interaction between YTHDF1 and Biotinylated ssDNA probe with m6-A modification
Readout
HTRF
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| ssDNA probe with m6-A modification (5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’) |
188 nM |
| Tegaserod | >100 μM |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Reference competition inhibition of YTHDC1 and Biotinylated ssDNA probe with m6-A modification binding interaction
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