YTHDC2 Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.
Target Overview
CAT#
YTHDC2
Enzyme
Human YTHDC2 (Full Length, FLAG tag at N-terminal)
Synonyms
YTH domain-containing protein 2, 3′-5′ RNA helicase YTHDC2
Scientific Information
Q9H6S0 (https://www.uniprot.org/uniprotkb/Q9H6S0/entry)
Assay Properties
Ligand
Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’
Reaction
Binding interaction between YTHDC2 and Biotinylated ssDNA probe with m6-A modification
Readout
HTRF
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| ssDNA probe with m6-A modification (5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’) |
53.4 nM |
| Tegaserod | >100 μM |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Reference competition inhibition of YTHDC2 and Biotinylated ssDNA probe with m6-A modification binding interaction
PDF