YTHDF1 Reader Domain Assay Service

YTHDF1 Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.

Target Overview
CAT#

YTHDF1

Enzyme

Human YTHDF1 (Full Length, FLAG tag at N-terminal)

Synonyms

YTH domain-containing family protein 1, C20orf21, DF1, Dermatomyositis associated with cancer putative autoantigen 1, DACA1

Scientific Information

Q9BYJ9 (https://www.uniprot.org/uniprotkb/Q9BYJ9/entry)

Assay Properties
Ligand

Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’

Reaction

Binding interaction between YTHDF1 and Biotinylated ssDNA probe with m6-A modification

Readout

HTRF

Reference Compound IC50s
Compounds IC50
ssDNA probe with m6-A modification
(5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’)		 64 nM
Tegaserod 54.4 μM
Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference competition inhibition of YTHDF1 and Biotinylated ssDNA probe with m6-A modification binding interaction

Reference competition inhibition of YTHDF1 and Biotinylated ssDNA probe with m6-A modification binding interaction

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