YTHDF2-YTH Reader Domain Assay Service

YTHDF2-YTH Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound biochemical binding to the target.

Target Overview
CAT#

YTHDF2-YTH Domain

Enzyme

Human YTHDF2 (401-554aa, N-terminal 6×His tag and a C-terminal Flag tag)

Synonyms

YTH domain-containing family protein 2, HGRG8, Renal carcinoma antigen NY-REN-2, CLL-associated antigen KW-14, High-glucose-regulated protein 8

Scientific Information

Q9Y5A9 (www.uniprot.org/uniprotkb/Q9Y5A9)

Assay Properties
Ligand

Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’

Reaction

Binding interaction between YTHDF2 and Biotinylated ssDNA probe with m6-A modification

Readout

HTRF

Reference Compound IC50s
Compounds IC50
ssDNA probe with m6-A modification
(5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’)		 47.9 nM
Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference competition inhibition of YTHDF2-YTH and Biotinylated ssDNA probe with m6-A modification binding interaction

Reference competition inhibition of YTHDF2-YTH and Biotinylated ssDNA probe with m6-A modification binding interaction

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