YTHDF2 Reader Domain Assay Service

YTHDF2 Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.

Target Overview

CAT#

YTHDF2

Enzyme

Human YTHDF2 (Full Length, FLAG tag at N-terminal)

Synonyms

YTH domain-containing family protein 2, HGRG8, Renal carcinoma antigen NY-REN-2, CLL-associated antigen KW-14, High-glucose-regulated protein 8

Scientific Information

Q9Y5A9 (www.uniprot.org/uniprotkb/Q9Y5A9)

Assay Properties

Ligand

Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’

Reaction

Binding interaction between YTHDF2 and Biotinylated ssDNA probe with m6-A modification

Readout

HTRF

Reference Compound IC50s

Compounds	IC50
ssDNA probe with m6-A modification (5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’)	59.7 nM
Tegaserod	91 μM

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference competition inhibition of YTHDF2 and Biotinylated ssDNA probe with m6-A modification binding interaction

PDF