YTHDF3-YTH Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound biochemical binding to the target.
Target Overview
CAT#
YTHDF3-YTH Domain
Enzyme
Human YTHDF3 (407-560aa, N-terminal 6×His tag and a C-terminal Flag tag)
Synonyms
YTH domain-containing family protein 3
Scientific Information
Q7Z739 (www.uniprot.org/uniprotkb/Q7Z739)
Assay Properties
Ligand
Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’
Reaction
Binding interaction between YTHDF3 and Biotinylated ssDNA probe with m6-A modification
Readout
HTRF
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| ssDNA probe with m6-A modification (5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’) |
78.1 nM |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Reference competition inhibition of YTHDF3-YTH and Biotinylated ssDNA probe with m6-A modification binding interaction
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