Protein Detection Methods

At Reaction Biology, protein detection supports biomarker discovery, monitoring of pharmacodynamic markers, and MoA analysis. We offer two methods for protein analysis:

  1. ELISA (enzyme-linked immunosorbent assay) is a high-throughput method to quantify proteins in cells or other biological samples, including liquids. The protein of interest is detected with specific antibodies and quantified with high accuracy.
  2. Western Blot Services enable the detection of proteins in biological samples based on specific antibodies and the molecular size of the protein. Our Western Blot Service provides qualitative and quantitative means to investigate the modulation of protein expression or post-translational modifications.

In addition, we also offer multiplexing methods for protein science including MSD and flow cytometry.

In most cases, Western Blot and ELISA studies are custom-tailored to the specific needs of the client. Please reach out to have a conversation with our scientists today.

Additional Information about Protein Detection Methods

ELISA Principle

ELISA is well established at Reaction Biology for quantification of proteins in a high-throughput format.

ELISA principle

The target protein can be quantified from liquid samples such as blood plasma or cell culture medium or solid samples including tissues or cultured cells. After lysis, the lysate is applied to multi-well plates prepared with target-specific capture antibodies immobilized on the plastic surface. The antibody captures the target proteins which in turn are detected by target-specific detection antibodies that are labeled with a fluorochrome for quantification.

Western Blot Principle

Our Western Blot Service is a highly sensitive low throughput method for protein identification via antigen-specific antibodies and molecular weight.

Western Blot Principle

In the first step, the proteins are solubilized via a detergent from tissue or cells. Next, the proteins are separated by their molecular size via SDS-PAGE before being transferred onto a membrane. The proteins are detected by specific antibodies labeled with fluorochromes quantified with a near-infrared imager.

The more sensitive enhanced chemiluminescence (ECL)-based imaging can be used as an alternative detection method.