Additional Information about Protein Detection Methods
ELISA is well established at Reaction Biology for quantification of proteins in a high-throughput format.
The target protein can be quantified from liquid samples such as blood plasma or cell culture medium or solid samples including tissues or cultured cells. After lysis, the lysate is applied to multi-well plates prepared with target-specific capture antibodies immobilized on the plastic surface. The antibody captures the target proteins which in turn are detected by target-specific detection antibodies that are labeled with a fluorochrome for quantification.
Our Western Blot Service is a highly sensitive low throughput method for protein identification via antigen-specific antibodies and molecular weight.
In the first step, the proteins are solubilized via a detergent from tissue or cells. Next, the proteins are separated by their molecular size via SDS-PAGE before being transferred onto a membrane. The proteins are detected by specific antibodies labeled with fluorochromes quantified with a near-infrared imager.
The more sensitive enhanced chemiluminescence (ECL)-based imaging can be used as an alternative detection method.