List of COVID-19 Protease Assays (8)
|ACE2||angiotensin I converting enzyme 2||Data sheet|
|Cathepsin B||APP secretase, Cathepsin B1, CATB, CTSB, CPSB||Data sheet|
|Cathepsin L||CTSL, CTSL1||Data sheet|
|Furin||FUR, PACE, PCSK3||Data sheet|
|Mpro||3CLpro, main protease||Data sheet|
|PLpro||NSP3, Papain-Like cysteine protease||Data sheet|
|TMPRSS2||Transmembrane protease serine 2, PRSS10||Data sheet|
|TMPRSS6||Transmembrane protease serine 6, matriptase 2||Data sheet|
See our full portfolio on our Protease Assay webpage.
SARS-CoV-2 Protease Assay Details
The papain-like protease (PLpro) is responsible for cleavage of the coronavirus polyprotein necessary for virus assembly. PLpro also suppresses the host innate immune through the reversal of ubiquitination and ISGylation events. We offer the investigation of the proteolytic activity of PLpro for deubiquitination and for proteolysis of a peptide.
The PLpro assay allows for screening compounds using a fluorogenic substrate, either Z-RLRGG-AMC or Ubiquitin-AMC. PLpro cleaves the substrate for the release of AMC and the resulting fluorescence can be quantified with a luminometer for readout of PLpro enzymatic activity.