PDE5A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE5A
Enzyme
Human PDE5A
Synonym
cGMP-specific 3′,5′-cyclic phosphodiesterase, CGB-PDE
Scientific Information
O76074 (http://www.uniprot.org/uniprot/O76074)
Assay Properties
Substrate
1 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Methoxyquinazoline | 1790 |
| Sildenafil | 9.8 |
| Trequinsin | 10900 |
| IBMX | 84600 |
| Zaprinast | 3120 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE5A
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