PDE Assay Services

Phosphodiesterase (PDE) screening is performed with an activity assay format based on the hydrolysis of cyclic AMP and cyclic GMP. Inhibition of PDEs prevents the regulation of these second messengers and was found useful in the treatment of a variety of conditions including pulmonary hypertension, acute refractory cardiac failure, erectile dysfunction, etc.

The activity of PDEs is measured with the Transcreener AMP2/GMP2 FP PDE assay platform (BellBrook labs)
Low and large scale screening, as well as high-throughput PDE screening options, are available
Custom-tailored PDE assay development possible

At Reaction Biology, we offer PDE screening services for pharma, biotech, and academic institutions with a commitment to quality data and quality service. Our global business development team is available to discuss your PDE assay needs with you today.

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List of PDE Assays (16 total)

Target	Synonyms	UniProt	Data
PDE10A	cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A	Q9Y233	Data sheet
PDE1A	Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A	P54750	Data sheet
PDE1B	Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1B	Q01064	Data sheet
PDE1C	Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1C	Q14123	Data sheet
PDE2A	cGMP-dependent 3′,5′-cyclic phosphodiesterase	O00408	Data sheet
PDE3A	cGMP-inhibited 3′,5′-cyclic phosphodiesterase A	Q14432	Data sheet
PDE3B	cGMP-inhibited 3′,5′-cyclic phosphodiesterase B	Q13370	Data sheet
PDE4A	cAMP-specific 3′,5′-cyclic phosphodiesterase 4A	P27815	Data sheet
PDE4B	cAMP-specific 3′,5′-cyclic phosphodiesterase 4B	Q07343	Data sheet
PDE4C	cAMP-specific 3′,5′-cyclic phosphodiesterase 4C	Q08493	Data sheet
PDE4D	cAMP-specific 3′,5′-cyclic phosphodiesterase 4D	Q08499	Data sheet
PDE5A	cGMP-specific 3′,5′-cyclic phosphodiesterase	O76074	Data sheet
PDE7A	High affinity cAMP-specific 3′,5′-cyclic phosphodiesterase 7A	Q13946	Data sheet
PDE7B	cAMP-specific 3′,5′-cyclic phosphodiesterase 7B	Q9NP56	Data sheet
PDE8A	High affinity cAMP-specific and IBMX-insensitive 3′,5′-cyclic phosphodiesterase 8A	O60658	Data sheet
PDE9A	High affinity cGMP-specific 3′,5′-cyclic phosphodiesterase 9A	O76083	Data sheet

PDE Screening Details

Assay Format

PDE converts cAMP or cGMP to AMP or GMP which displace a fluorescent tracer from an antibody selective for AMP and GMP resulting in a reduction of the fluorescence polarization signal.

Assay Setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format.

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.

Please see our FAQ page to access our assay condition form (US facility).

Monthly Screening Schedule

On a monthly basis, Reaction Biology performs compound screening on the full phosphodiesterase panel.

Please see the upcoming screening dates here.

PDE Assay Services

List of PDE Assays (16 total)

PDE Screening Details

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