KDM5C Demethylase Assay for compound screening and profiling via HTRF-based quantification of enzyme activity.
Target Overview
CAT#
KDM5C
Enzyme
Human KDM5C
Synonym
Lysine-specific demethylase 5C, Jumonji/ARID domain-containing protein 1C, JARID1C
Substrates
B-H3(1-21)-K4Me3, α-KG
Assay Properties
Reaction
H3(1-21)-K4Me3 + α-KG + O₂ = H3(1-21)-K4Me2 + succinate + CO₂ + formaldehyde
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| GSK-J1 | 120 |
| 2,4-PDCA | 890 |
Scientific Information
P41229 (http://www.uniprot.org/uniprot/P41229)
Screening Location
Malvern, USA
Reference compound inhibition of KDM5C
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