LSD1 Demethylase Assay for compound screening and profiling via Amplex Red-based quantification of enzyme activity.
Target Overview
CAT#
LSD1
Enzyme
Human LSD1
Synonym
Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, AOF2, KDM1, KIAA0601, KDM1A
Substrate
Histone H3 peptide (1-21) K4me2, 10 μM
Assay Properties
Reaction
Histone [methyl]-L- Lysine + O₂ + LSD1 + FAD = Histone L- Lysine + H₂O₂H₂O₂ + Amplex Red® + HRP = Resorufin (Ex/Em=535/590)
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Tranylcypromine | 2,300 |
Scientific Information
O60341 (http://www.uniprot.org/uniprot/O60341)
Screening Location
Malvern, USA
Reference compound inhibition of LSD1
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