ROS1(1883-2347) NanoBRET Kinase Assay

ROS1(1883-2347) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview
Target

ROS1(1883-2347)

HGNC Symbol

ROS1

Synonym

ROS, MCF3

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties
Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-ROS1(1883-2347) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-9 and then treated with reference compound CC1 pan-Kinase Inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
CompoundIC50 (M)
CC1 pan-Kinase Inhibitor 8.69E-008
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.

Reference compound IC50 for ROS1(1883-2347)