A20 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
A20/TNFAIP3
Enzyme
Human A20/TNFAIP3
Synonym
Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, OTUD7C, TNAP3
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of Ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 41.8 |
| Ub Aldehyde | 5.64 |
| UCHL1 I 2 | 65.8 |
Scientific Information
P21580 (http://www.uniprot.org/uniprot/P21580)
Screening Location
Malvern, USA
Reference Compound IC50 for A20/TNFAIP3
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