A20 Ubiquitin-Proteasome Pathway Assay Service

A20 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

A20/TNFAIP3

Enzyme

Human A20/TNFAIP3

Synonym

Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, OTUD7C, TNAP3

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of Ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 41.8
Ub Aldehyde 5.64
UCHL1 I 2 65.8
Scientific Information

P21580 (http://www.uniprot.org/uniprot/P21580)

Screening Location

Malvern, USA

Reference Compound IC50 for A20/TNFAIP3

Reference Compound IC50 for A20/TNFAIP3