List of Proteasome and DUB Assays (14)
|20S Proteasome||-||Data sheet|
|A20||TNFAIP3, TNF alpha induced protein 3||Data sheet|
|Ataxin 3||ATXN3||Data sheet|
|BAP1||BRCA1 associated protein 1||Data sheet|
|NEDP1||Sentrin/SUMO-specific peptidase 8||Data sheet|
|SENP1||Sentrin/SUMO-specific peptidase 1||Data sheet|
|SENP2||Sentrin/SUMO-specific peptidase 2||Data sheet|
|UCHL1||Ubiquitin C-terminal hydrolase L1||Data sheet|
|UCHL3||Ubiquitin C-terminal hydrolase L3||Data sheet|
|USP10||Ubiquitin-specific peptidase 10||Data sheet|
|USP14||Ubiquitin-specific peptidase 14||Data sheet|
|USP2||Ubiquitin-specific peptidase 2||Data sheet|
|USP5||Ubiquitin-specific peptidase 5||Data sheet|
|USP8||Ubiquitin-specific peptidase 8||Data sheet|
Deubiquitination Assay Details
Fluorescent Ubiquitin-AMC is a substrate containing a fluorophore, 7-amido-4-methyl coumarin (AMC), that is quenched when ubiquitylated. After cleavage by a deubiquitinase, AMC is released and its fluorescence in the absence and presence of a deubiquitinase inhibitor can be measured.
Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.
Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format.
Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.
Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.
Screening facility: This assay is performed at our screening facility in Malvern, PA, US.
Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.