ABL1 (H396P) NanoBRET Kinase Assay

ABL1 (H396P) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview


ABL1 (H396P)

HGNC Symbol

ABL1 (H396P)


ABL; JTK7; P150; c-ABL; v-ABL

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-ABL1(H396P) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-4 and then treated with reference compound Dasatinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Dasatinib 2.056e-009

Screening Location

Malvern, USA

Reference compound IC50 for ABL1 (H396P)