AR

Nuclear receptor assay, cell-based luciferase reporter assay

Target

Androgen receptor (AR)

HGNC symbol

AR

Synonyms

AIS, NR3C4, SMAX1, HUMARA

Cell line

Human AR Reporter Cell Line

Assay description
  • assay format: 96-well format
  • principle: The AR Reporter Cells include the luciferase reporter gene functionally linked to a bona fide AR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in AR activity (INDIGO biosciences).
  • readout: receptor activity
AR agonist assay example

 

Human AR Reporter cells were plated in a 96-well plate and then stimulated with various concentrations of reference agonist 6a-FIT-R  for 24 hours. The luminescence signal was measured with an EnVision 2104 Multilabel Reader. AR activation dose-response curve was plotted using the GraphPad Prism program. 

 

 

AR antagonist example

 

Human AR Reporter cells were plated in a 96-well plate and then stimulated with various concentrations of reference antagonist Enzalutamide and a fixed concentration of agonist 6a-FIT-R  for 24 hours. The luminescence signal was measured with an EnVision 2104 Multilabel Reader. AR activation dose-response curve was plotted using the GraphPad Prism program.