Androgen Receptor Assay Service

AR Nuclear Receptor Assay for compound screening in intact cells via luciferase reporter assay to quantify nuclear receptor activity.
Target Overview

Target

Androgen receptor (AR)

HGNC symbol

AR

Synonyms

AIS, NR3C4, SMAX1, HUMARA

Cell line

Human AR Reporter Cell Line

Assay Properties

Assay description
  • assay format: 96-well format
  • principle: The AR Reporter Cells include the luciferase reporter gene functionally linked to a bona fide AR–responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in AR activity (INDIGO biosciences).
  • readout: receptor activity

Further information

More information can be found on our website Nuclear Receptor Assay Services.

AR agonist assay example

 

Human AR Reporter cells were plated in a 96-well plate and then stimulated with various concentrations of reference agonist 6a-FIT-R  for 24 hours. The luminescence signal was measured with an EnVision 2104 Multilabel Reader. AR activation dose-response curve was plotted using the GraphPad Prism program. 

 

 

AR antagonist example

 

Human AR Reporter cells were plated in a 96-well plate and then stimulated with various concentrations of reference antagonist Enzalutamide and a fixed concentration of agonist 6a-FIT-R  for 24 hours. The luminescence signal was measured with an EnVision 2104 Multilabel Reader. AR activation dose-response curve was plotted using the GraphPad Prism program. 

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