Ataxin-3 Ubiquitin-Proteasome Pathway Assay Service

Ataxin-3 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

Ataxin-3/ATXN3

Enzyme

Human Ataxin-3/ATXN3

Synonym

Machado-Joseph disease protein 1, Spinocerebellar ataxia type 3 protein,ATX3, MJD, MJD1, SCA3

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of Ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 2 153
UCHL3 I 298
Scientific Information

P54252 (http://www.uniprot.org/uniprot/P54252)

Screening Location

Malvern, USA

Reference Compound IC50 for Ataxin-3/ATXN3

Reference Compound IC50 for Ataxin-3/ATXN3