BAP1 Ubiquitin-Proteasome Pathway Assay Service

BAP1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

BAP1

Enzyme

Human BAP1

Synonym

Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1,Cerebral protein 6, KIAA0272, hucep-6)

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of Ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 180
UIPase I >300
UI1 >300
Scientific Information

Q92560 (http://www.uniprot.org/uniprot/Q92560)

Screening Location

Malvern, USA

Reference Compound IC50 for BAP1

Reference Compound IC50 for BAP1