Surface Plasmon Resonance (SPR) technique for evaluating direct binding of BRD4-1 to molecular inhibitors
Target Overview
Target
BRD4-1
HGNC symbol
BRD4-1
Synonyms
Bromodomain-containing protein 4 domain 1, Protein HUNK1
Enzyme
Human BRD4-1
Uniprot page
O60885 (https://www.uniprot.org/uniprot/O60885)
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Close affinity, different kinetics:
JQ1 and Bromsporine both showed sub-nano molar binding affinity to the BRD4-1. Bromosporine dissociates about 10 times faster than JQ1, which reflects a short on-target residence time.
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