BRD4-2 NanoBRET TE Intracellular Assay

BRD4-2 NanoBRET Target Engagement Intracellular Assay for screening of compounds binding to the target, competitive binding assay format

Target Overview



HGNC Symbol

BRD4 (BD2)



Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET Intracellular Target Engagement Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-BRD4 (BD2) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET BRD Tracer and then treated with reference compound IBET-151 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.


Binding of compound to target

Reference Compound IC50s
Compounds IC50 (M)
IBET-151 2.257e-006

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Cell-based Epigenetic Assays

Reference compound IC50 for BRD4 (BD2)

Reference compound IC50 for BRD4 BD2