CAMKK2(165-445) NanoBRET Kinase Assay

CAMKK2(165-445) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview

Target

CAMKK2(165-445)

HGNC Symbol

CAMKK2

Synonyms

CAMKK, CAMKKB, KIAA0787, MGC15254

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-CAMKK2(165-445) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-9 and then treated with reference compound Staurosporine for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target

Reference Compound IC50s

Compounds	IC50 (M)
Staurosporine	4.718e-010

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.

Reference compound IC50 for CAMKK2(165-445)

PDF