CDK13+Cyclin K NanoBRET Kinase Assay

CDK13+Cyclin K NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview
Target

CDK13+Cyclin K

HGNC Symbol

CDK13/CCNK complex

Synonyms

CDC2L; CDC2L5; CHED

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties
Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing CDK13-SmBiT(q)_LgBiT-CCNK BiBiT vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-12 and then treated with reference compound THZ531 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
THZ531 4.781e-007
Screening Location

Malvern, PA, USA

Reference Compound IC50 for CDK13-SmBiT(q)_LgBiT-CCNK
Reference compound IC50 for CDK13+Cyclin K