CDK13+Cyclin K NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview
Target
CDK13+Cyclin K
HGNC Symbol
CDK13/CCNK complex
Synonyms
CDC2L; CDC2L5; CHED
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Properties
Assay Format
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
Assay Protocol
HEK293 cells transiently expressing CDK13-SmBiT(q)_LgBiT-CCNK BiBiT vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-12 and then treated with reference compound THZ531 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Readout
Binding of compound to target kinase
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| THZ531 | 4.781e-007 |
Screening Location
Malvern, PA, USA
Reference Compound IC50 for CDK13-SmBiT(q)_LgBiT-CCNK
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