CDK15+Cyclin Y NanoBRET Kinase Assay

CDK15+Cyclin Y NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview

CDK15+Cyclin Y

HGNC Symbol

CDK15/CCNY complex



Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties
Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc- CDK15 Fusion Vector and Cyclin Y Vector were seeded into the wells of 384-well plates. The cells were pretreated with the NanoBRET Tracer K-10 and then treated with reference compound AT7519 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
AT7519 2.796e-007
Screening Location

Malvern, USA

Reference compound IC50 for CDK15+Cyclin Y