HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently co-expressing NanoLuc- CDK19 fusion vector and Cyclin C Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-8 and then treated with reference compound AST-487 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
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