CDK1+Cyclin B1

NanoBRET Target Engagement Intracellular Kinase Assay, Competitive binding assay format

Target

CDK1+Cyclin B1

HGNC Symbol

CDK1/CCNB1 complex

Synonyms

CDC2; p34

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc- CDK1 Fusion Vector and Cyclin B1 Vector were seeded into the wells of 384-well plates. The cells were pretreated with the NanoBRET Tracer K-10 and then treated with reference compound Dinaciclib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Dinaciclib 5.572e-007
Screening Location

Malvern, USA

Reference compound IC50 for CDK1+Cyclin B1